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Rabbit polyclonal antibody against CYGB conjugated to Biotin Isotype Note: IgG Host Note: Rabbit Conjugation Note: Biotin Reactivity Note: Human Application Note: ELISA
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CYGB mouse monoclonal antibody clone OTI6G6 HRP conjugated
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Image Search Results
Journal: Journal of Cellular and Molecular Medicine
Article Title: Cytoglobin promotes sensitivity to ferroptosis by regulating p53‐YAP1 axis in colon cancer cells
doi: 10.1111/jcmm.16400
Figure Lengend Snippet: CYGB promoted YAP1 expression and was involved in ferroptosis signalling pathway. A. Demonstration of significant changes in the expression of genes based on RNA‐seq in the CYGB‐overexpressing HCT116 cells compared to MOCK cells. B. The most twenty significantly enriched KEGG pathways based on CYGB overexpression. C. Immunoblotting analysis of YAP1, P‐YAP1 and ACSL4 in the MOCK‐ and CYGB‐overexpressing cells. GAPDH was used as a loading control. D. The comparison of obviously changed genes between RNA‐seq in present study and the colon cancer data from TCGA in Figure . E. The most twenty significantly enriched KEGG pathways based on overlapped genes in D. F, G. The list of obviously changed ferroptosis‐related genes between comparison of CYGB‐low and CYGB‐high expression in colon cancer from TCGA data (F) and RNA‐seq in present study (G). H, I. Gene set enrichment analysis (GSEA) indicates that high expression of CYGB is associated with the hippo (H) and ferroptosis signalling pathway (I) in the TCGA database
Article Snippet:
Techniques: Expressing, RNA Sequencing, Over Expression, Western Blot, Control, Comparison
Journal: Journal of Cellular and Molecular Medicine
Article Title: Cytoglobin promotes sensitivity to ferroptosis by regulating p53‐YAP1 axis in colon cancer cells
doi: 10.1111/jcmm.16400
Figure Lengend Snippet: CYGB promoted ferroptosis sensitivity is YAP1 dependent. A. Immunoblotting analysis of YAP1 and ACSL4 in HCT116 cells treated with siRNAs targeting YAP1 for 72 hours. B, C. Cellular (B) and lipid (C) ROS detection with DCFDA and C11‐BODIPY, respectively. D. Cell death analysis with propidium iodide (PI) staining by flow cytometry after the application of RSL3, with or without YAP1 siRNAs. Data represent the mean ± SD of three biological replicates. * P < 0.05, ** P < 0.05. E, F. Positive correlations between CYGB and YAP1 (E); and between CYGB and ACSL4 (F) mRNA expression in colon cancer from TCGA
Article Snippet:
Techniques: Western Blot, Staining, Flow Cytometry, Expressing
Journal: Journal of Cellular and Molecular Medicine
Article Title: Cytoglobin promotes sensitivity to ferroptosis by regulating p53‐YAP1 axis in colon cancer cells
doi: 10.1111/jcmm.16400
Figure Lengend Snippet: YAP1 was a downstream target of p53. A. Immunoblotting analysis of p53, P‐p53 and SLC7A11 in the MOCK‐ and CYGB‐overexpressing cells. B. Immunoblotting analysis of p53, YAP1 and SLC7A11 in the HCT116 cells treated with siRNAs targeting p53 for 72 hours. C. Transcriptional level of p53, SLC7A11 and YAP1 in the HCT116 cells treated with siRNAs targeting p53 for 72 hours. D. Effects on the lipid peroxidation after p53 knock‐down in the MOCK‐ and CYGB‐overexpressing HCT116 were assessed with C11‐BODIPY by flow cytometry. Data represent the mean ± SD of three biological replicates. * P < 0.05, ** P < 0.05. E. Correlations between CYGB and P53 mRNA expression in colon cancer from TCGA. F. A proposed working model of CYGB to promote sensitivity of ferroptosis. YAP1 was the downstream of p53 which was activated by CYGB to promote lipid peroxidation and ferroptosis
Article Snippet:
Techniques: Western Blot, Knockdown, Flow Cytometry, Expressing